Fig. 4
From: A new class of human CpG Island promoters with primate-specific repeats
Effects of spermatogenesis-associated reprogramming on the methylation levels of TR-CGIs. (A) Proportion of non-TR CGIs (red), ICRs (green), and TR-CGIs (blue) with differences in methylation between blood/saliva and semen. Values were determined by comparing the same samples from five different individuals. Average methylation levels in the blood and saliva (body fluids) are used as reference. (B) Most changes between blood/saliva and sperm for non-TR CGIs (red), ICRs (green) and TR-CGIs (blue) involve hypomethylation. (C–E) PCA analyses of the three different categories of CGIs based on their methylation levels in blood, saliva and semen. (F) Methylated fractions of TR-CGIs, non-TR CGIs and ICRs in sperm and MII oocytes. (G) TR-CGI methylation from gametes through preimplantation cleavage stages and post-implantation embryos. Values are methylated fractions of CGI CpGs. Seven TR-CGIs highlighted in yellow are methylated in all preimplantation stages. 32 TR-CGIs were examined. Green rectangles – low methylation. Salmon rectangles – high methylation. Data are taken from publicly available datasets: GSE49828, GSE51239.
