Fig. 4
From: Targeting oncofetal fibronectin and neuropilin-1 in solid tumors with PL2 peptide
PL2-NWs accumulate in glioblastoma lesions and colocalize with Fn-EDB and NRP-1. (A–C, upper row) PL2-functionalized NWs and (D–F, lower row) control NWs were injected i.v. at 7.5 mg/kg into mice bearing s.c. U87-MG glioblastoma xenografts. After 5 h of circulation, mice were perfused with PBS/DMEM, and tissues were harvested for ex vivo macroscopic imaging and fluorescence microscopy. (A, D) Ex vivo macroscopic Illumatool images of PL2- and control NWs in the green channel. The superimposed images of white light and green fluorescence channels are representative of three independent experiments. (B, C, E, F) Confocal microscopy images of NWs, Fn-EDB, and NRP-1 in tumor cryosections. PL2-NWs colocalized with Fn-EDB and NRP-1 (arrows), whereas (E, F) FAM-NWs showed only background accumulation. Tumor tissues were stained with rabbit anti-FAM (green), anti-Fn-EDB ScFv L19 (red), and rabbit anti-NRP-1 (red) antibodies. PL2-NWs (B, C) colocalize with Fn-EDB and NRP-1 (indicated by arrows), whereas control NWs (E, F) exhibit only background accumulation. Scale bar, 100 μm; the images are representative of 3 independent experiments.
