Fig. 5
From: Targeting oncofetal fibronectin and neuropilin-1 in solid tumors with PL2 peptide
Confocal fluorescence imaging of PL2-NW binding to human ovarian carcinoma cancer explants. Fresh surgical explants of human ovarian carcinoma were incubated with PL2-NWs and control NWs at 37 °C for 4 h. The tissue sections were then stained with rabbit anti-FAM primary antibodies (green) and Alexa Fluor 488-conjugated anti-rabbit secondary antibodies. The nuclei were counterstained with DAPI (blue). The arrows indicate PL2-NW signals at the tumor rim and extravasation into the tissue. (B) Quantification of FAM fluorescence signal from the confocal images using Fiji ImageJ. Error bars represent mean ± SEM (N = 3). Statistical analysis was performed using Student’s t-test; ** p < 0.01.
