Table 1 In vivo playoff auditioning of Fn-EDB-selected peptides in tumor xenograft models in mice.

From: Targeting oncofetal fibronectin and neuropilin-1 in solid tumors with PL2 peptide

Phage-displayed Peptides in

the “Playoff” Mix

Representation of the phage in tumors or in control brain tissue (fold over G7 control phage)

WT GBM

P3 stem cell-like

P13

U87-MG

PC3

Normal brain

Control

GGGGGGG (G7)

Control

1.0

1.0

1.0

1.0

1.0

1.0

Fn-EDB-selected

(round 5)

TKRKGKG

Clone-2

2.7

0.7

0.8

2.0

1.0

0.2

GLGGRRIKLKTS

Clone-3

0.8

0.7

1.3

0.6

1.5

0.1

GRRGRVIKLKTSEPPQ

Clone-4

0.8

0.5

1.9

0.8

1.3

0.3

KVKKRGA

Clone-17

1.5

0.3

1.6

1.0

0.7

0.1

RESRRGRVKLAAALE

Clone-33

4.0

0.6

1.0

1.2

1.3

0.2

TSKQNSR

Clone-46

11.6

32.2

4.8

19.0

4.5

0.4

CTVRTSADC

ZD2

4.5

0.7

3.1

2.1

1.7

0.2

  1. Significant values are in [bold].
  2. An equimolar mixture of Fn-EDB-selected phages was intravenously injected into mice bearing orthotopic WT-GBM, P3 stem cell-like, P13, and s.c. implanted U87-MG glioblastoma, or PC3 prostate carcinoma xenografts at a dose of 1 × 1010 pfu/mouse. After 2 h of circulation, background phages were removed by perfusion, and the representation of individual phages in tumor and control tissues was evaluated by Ion-Torrent high-throughput sequencing. Phages displaying PL2 (TSKQNSR) peptide showed the highest representation across tumor models tested in tumor tissue. The data represent the mean of 3 mice for each model.
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