Fig. 8 | Scientific Reports

Fig. 8

From: Sodium formate-induced mitochondrial impairment and cytotoxicity in neuronal cells reveal crucial pathogenic mechanisms underlying diabetic neuropathy and retinopathy

Fig. 8

Effect of SF on cellular ROS levels in HT22 cells. (A) Effect of SF on cellular ROS levels (fluorescence microscopy, scale bar = 25 μm). Cells were treated with SF (12.5–100 mM) for 24, 48 and 72 h, and then stained with DCFH-DA for imaging analysis. Quantitative values for A are shown in Supplementary Fig. S11A‒S11C. (B) Time-dependent effect (24–96 h) of 25 mM SF on cellular ROS levels (fluorescence microscopy, scale bar = 25 μm). Quantitative values for B are shown in Supplementary Fig. S11D (C–G). Time-dependent effect of SF on cellular ROS levels (flow cytometry) following treatment with 25 mM SF for 24 h (C), 48 h (D), 72 h (E), 96 h (F), and 120 h (G). The left panels are the histograms, and the right panels are the corresponding quantitative intensity values (the value for the control group is labeled on top of the bar). Each quantitative value is the mean ± S.D (n = 3). * and ** denote for P < 0.05 and P < 0.01, respectively.

Back to article page