Fig. 1

The immunofluorescence of smooth muscle alpha-actin-2 (ACTA2), calponin 1 (CNN1) and the smooth muscle myosin heavy chain in primary smooth muscle cells isolated from the human aorta. (A) SMCs from the tunica media were fixed and incubated with ACTA2 antibody (red). (B) SMCs from the tunica media CNN1 were fixed and incubated with CNN1 antibody (green). (C) SMCs from the tunica intima were fixed and incubated with ACTA2 antibody (red). (D) SMCs from the tunica intima were fixed and incubated with MYH11 antibody (green). Nuclei were stained with DAPI (blue) (A–D). The scale bar is 25 microns (A–D).