Fig. 3

Graph of the mean growth ± SD of cryopreserved larvae and control larvae throughout larval development from 24 hpf (hours post-fertilization) until 40 dpf * (days post-fertilization) (40d * last modification protocol), 48 h post-thawing (A). For each parameter, 35 larvae were measured (n = 3). Graph of the percentage of larval normality ± SD of cryopreserved larvae and their controls throughout larval development from 24 hpf until 40 dpf * (40d* last modification protocol), 48 h post-thawing (B). For each parameter, 100 larvae were examined (n = 3). Asterisks represent significant differences compared with the control without cryoprotectant; * indicates p ≤ 0.05. Graph of % survival of cryopreserved larvae and their controls throughout larval development from 24 hpf to 40 dpf * (40d* last modification protocol), T1 (0 h post-thawing), T2 (24 h post-thawing) and T3 (48 h post-thawing), analyzed via the fluorescent live-dead dye (C). Images of control larvae obtained via light microscopy (D) and images of cryopreserved larvae after 72 h stained with propidium iodide and Hoechst, obtained via fluorescence microscopy, along different stages of larval development of M. galloprovincialis.