Fig. 4
Dose‒response curve of 40-day-old M. galloprovincialis juveniles exposed to different concentrations of EG. For each parameter, 100 larvae were measured (n = 4) (A). Graph of % survival of cryopreserved juveniles at 40 days post-fertilization at three time points, T1 (0 h post-thawing), T2 (24 h post-thawing) and T3 (48 h post-thawing), analyzed via the fluorescent live-dead dye for two concentrations of cryoprotectant, EG10% or 12% with a TRE of 0.4 M, n = number of juveniles analyzed per treatment as present in 1 mL of sample (B). The thick arrow shows particulate material expelled by the juvenile after thawing. Images of cryopreserved juveniles fed with EG10% TRE 0.4 M and of cryopreserved juveniles fed EG10% TRE 0.4 M at three time points, (0 h post-thawing), T2 (24 h post-thawing) and T3 (48 h post-thawing), with propidium iodide and Hoechst dye (C).
