Fig. 5
Graph of the mean growth ± SD of cryopreserved feed 45 hpf juvenile and control 45hpf juvenile 48 h post-thawing (A). For each parameter, 35 larvae were measured (n = 3). Graph of the percentage of larval normality ± SD of cryopreserved feed 45hpf juvenile and control 45 hpf juvenile 48 h post-thawing (B) For each parameter, 100 larvae were examined (n = 3). There were no significant differences (p ≤ 0.05) compared with the control without the cryoprotectant. Graph of % survival of cryopreserved juveniles at 45 dpf cryopreserved with EG 12%+TRE 0.4 M at three time points, T1 (24 h after thawing), T2 (48 h after thawing) and T3 (72 h after thawing), analyzed via the fluorescent live-dead dye EG 12%+TRE 0.4 M, n = number of juveniles analyzed per treatment as present in 1 mL of sample (C). Images of cryopreserved juveniles fed EG12% +TRE 0.4 M and of cryopreserved juveniles fed EG12%+TRE 0.4 M at three time points, T1 (24 hpt), T2 (48 hpt) and T3 (72 hpt), with propidium iodide (red, dead cells) and Hoechst (blue, active DNA) dye (D). The thin arrow points to the edge of the mantle, the thick arrow to the foot, the caret to the digestive tract and the asterisk to the gills.
