Fig. 2 | Scientific Reports

Fig. 2

From: Proteomic analysis of brain and spinal cord tissue reveals distinct immune and mitochondrial processes between human and mouse ALS models

Fig. 2

Proteomic signatures across ALS tissue are highly overlapping. (A) Schematic of experimental design. The motor cortex and cervical, lumbar and thoracic spinal cord tissue was obtained from 5 control and 10 ALS cases. Proteins were then extracted for TMT proteomics. (B) Principal component analysis plot coloured by condition and tissue type. (C) Partial Least Squares Discriminant analysis (PLS-DA) plot classed and coloured by condition and tissue type. (D-F) Heatmap showing the normalized, log2 and scaled protein expression values across tissue and condition. Hierarchical clustering was performed on rows (tissue) and columns (proteins). (E) Upset plot showing the number of unique and common significantly differentially expressed proteins (DEPs) in ALS versus control samples, for each tissue type. (F) Volcano plots showing up- and down-regulated proteins in all control versus all ALS tissue. (G) Top 10 upregulated proteins in each ALS tissue type compared to controls.

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