Table 1 Plackett–Burman design for screening culture conditions for variables.
Independent Variables | Media Components (g/L) | Levels | |
|---|---|---|---|
−1 | + 1 | ||
A | Inositol | 5 | 20 |
B | Starch | 5 | 20 |
C | L-asparagine | 2 | 8 |
D | K2HPO4 | 1 | 3 |
E | Yeast Extract | 2 | 8 |
F | FeSO40.7 H2O | 0.0002 | 0.003 |
F | ZnSO40.7 H20 | 0.0002 | 0.003 |
H | pH | 3 | 12 |
I | Temperature°C | 25 | 45 |
J | Incubation Period (Days) | 3 | 14 |