Fig. 2
From: Shadowless amyloid imaging with quantitative birefringence contrast
Birefringence and dichroism imaging of renal arteries. (a) HE-stained image of adjacent pathology sections. (b) Congo red stained image (without analyzer). (c) Crossed Nicols observation of Congo red staining at θ = 0°. 2D distribution of (d) retardance, (e) azimuthal angle, and (f) dichroism, calculated using the intensity distribution of the green band. (g) Crossed Nicols images for polarizer angles θ = 0°, 30°, 60°, 90°, 120°, and 150°, showing characteristics birefringence shifting from yellow to green as the polarization shadow changes. 2D distribution of (h) retardance, (i) azimuthal angle, and (j) dichroism within the white frame shown in (c). Scale bars of (a–f) and (g–j) indicate 50 μm and 10 μm, respectively.
