Fig. 3
From: Shadowless amyloid imaging with quantitative birefringence contrast

Comparison of retardance and agreement. (a) Retardance image of amyloid deposits stained with Congo red. (b) Image observed under crossed Nicols with the polarizers at θ = 0° and (c) θ = 45°, showing Congo red staining. (d–g) Shadowless amyloid images averaged at N = 2, 4, 8, and 18, respectively, for different angles in a crossed Nicols arrangement. (h) Results evaluating the agreement between the intensity and retardance distributions above. Scale bars indicate 10 μm.