Fig. 3 | Scientific Reports

Fig. 3

From: Shadowless amyloid imaging with quantitative birefringence contrast

Fig. 3

Comparison of retardance and agreement. (a) Retardance image of amyloid deposits stained with Congo red. (b) Image observed under crossed Nicols with the polarizers at θ = 0° and (c) θ = 45°, showing Congo red staining. (d–g) Shadowless amyloid images averaged at N = 2, 4, 8, and 18, respectively, for different angles in a crossed Nicols arrangement. (h) Results evaluating the agreement between the intensity and retardance distributions above. Scale bars indicate 10 μm.

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