Fig. 4

H&E-stained sections of tissue-engineered oral mucosa (TEOM) treated with conditioned medium derived from liquid-PRF after 4 and 10 days. The upper purple region of each image is the epithelium comprised of FN6/TERT cells, while the lower pink region is the fibroblast populated lamina propria. Representative images of TEOM sections cultured in conditioned medium containing PRF 0%, PRF 10%, PRF 20%, PRF 50% and FBS 10% after 4 and 10 days (a). The epithelium thickness (b) and the metabolic activity (c) of TEOM on day 4 and day 10 of culture (N = 3 n = 1). Mean ± standard deviation (S.D). Statistical analysis was performed using an ordinary one-way ANOVA followed by Dunnett’s multiple comparison against the control (PRF 0%) at each time point. Scale bar = 100 μm. Abbreviations; FBS, foetal bovine serum; PRF, platelet-rich fibrin.