Fig. 2

Schematic representation of the T-DNA cassette of (a) construct pY, (b) construct pI and (c) construct pW used for the transformation of BY-2 cells. Regions are represented as follows: left border (LB), nopaline synthase terminator (tNOS), kanamycin resistance marker (NeoR/KanR), nopaline synthase promoter (pNOS), matrix attachment regions (MAR), Cauliflower Mosaic Virus 35 S promoter (CaMV 35 S), site-specific recombination site (attB1 and attB2), transit peptide (TP), phytoene synthase coding sequence (AY324431), phytoene desaturase coding sequence (D90087), β-carotene ketolase coding sequence, 35 S terminator (T35S), right border (RB). All dark lines are nucleotide sequences without relevant features.