Fig. 3

Phenotype of Nicotiana tabacum BY-2 cell lines compared to the wild-type. (a) Classification of BY-2 cell lines containing the heterologous psy, crtI, and/or crtW genes resulting from the single and combinatorial transformations with the pY, pI, and/or pW vectors. (b) Pale, yellow or pink-orange pigmented tobacco BY-2 calli. (c) BY-2 cultured cells, the WT and the highest yielding cell lines for the production of xanthophylls, astaxanthin, and canthaxanthin.