Fig. 1
Cell counts of myeloid cells, monocytes and neutrophils, and mean intensity fluorescence (MFI, expression) of CCR2 in the surface of the monocytes measured by flow cytometry 24 h after HCl administration in the (A) bronchoalveolar lavage (BAL), (B) circulation (blood) and (C) in the femur’s bone marrow. CCL2 concentration 24 h after HCl administration in (D) BAL, (E) plasma, and (F) bone marrow lavage (BM-lavage). (G) Proinflammatory mRNA gene expression measured by real time qPCR in lung tissue homogenates. (H) IL1β and IL6 concentration was measured in bronchoalveolar lavage (BAL). Data shown by mean ± SEM. Exact p values or *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 vs the control group; #p ≤ 0.05 vs the HCl/LPS group are shown in the graphs to the respective compared group (n = 5–6 per group). After testing data for normality, one-way-ANOVA followed by Neuman–Keuls post hoc test or In Kruskal–Wallis test followed by Dunn’s post hoc test was performed.
