Fig. 4

Expression of pro-inflammatory and recruitment chemokines in lung tissue after 72 h of the first insult. (A) CCL2 concentration in bronchoalveolar lavage (BAL), bone marrow lavage (BM-lavage) and plasma, (B) Proinflammatory and chemokine ligands mRNA gene expression measured by real time qPCR in lung tissue homogenates, (C) IL1β and (D) IL6 concentration in BAL. (E) Chemokine receptors mRNA gene expression measured by real time qPCR in lung tissue homogenates. Data shown by mean ± SEM. Exact p values or *p ≤ 0.05 vs the control group; #p ≤ 0.05 vs the HCl/LPS group (n = 5–8 per group). After testing data for normality, one-way-ANOVA followed by Neuman–Keuls post hoc test or In Kruskal–Wallis test followed by Dunn’s post hoc test was performed.