Fig. 3 | Scientific Reports

Fig. 3

From: CXCL12 chemokine dimer signaling modulates acute myelogenous leukemia cell migration through altered receptor internalization

Fig. 3

Differential CXCR4 C-terminal phosphorylation in response to CXCL12 variants. (A) HeLa cells transiently transfected with HA-CXCR4-YFP were stimulated with vehicle, (B) CXCL12-WT, (C) CXCL12-LM, or (D) CXCL12-LD. The far left panels show visualization of YFP-CXCR4 and the middle left panel is visualization of the phosphorylated Ser324/325 residues on CXCR4. Co-localization of the YFP-CXCR4 and phosphorylated CXCR4 appear yellow in the merge panel on the middle right. Differential image contrast (DIC) images are shown on the far right panels. Shown are the representative micrographs from three independent experiments. Bars = 20 μm.

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