Fig. 6 | Scientific Reports

Fig. 6

From: CXCL12 chemokine dimer signaling modulates acute myelogenous leukemia cell migration through altered receptor internalization

Fig. 6

CXCR4 surface upregulation seen with AMD3100 and BL-8040 but not CXCL12-LD. (A) U937 cells were treated with either 1 µM AMD3100, 100 nM BL-8040, or 100 nM CXCL12-LD and immunostained for surface CXCR4 with the 1D9 clone. Mean fluorescent intensity (MFI) values were normalized against vehicle-treated (PBS) U937 cells stained for surface CXCR4. Treatment was for 30 min or (B) 24 h. (C) CXCR4 inhibitor treatment of AML-293 cells for 30 min or (D) 24 h under the same conditions seen in 6 A and 6B. * P ≤ 0.05 by one-way ANOVA with Tukey’s multiple comparisons test.

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