Table 1 Expression profiles of candidate reference genes in transcriptome sequencing.
NO | Gene id | FPKM | Average | SD | CV | Swissprot annotation | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
CK | T1 | T2 | T3 | T4 | T5 | ||||||
1 | Cluster-37.23244 | 66.303 | 62.193 | 64.487 | 64.830 | 67.010 | 64.473 | 64.883 | 1.679 | 0.026 | E3 ubiquitin-protein ligase UPL3 OS = Arabidopsis thaliana OX = 3702 GN = UPL3 PE = 1 SV = 1 |
2 | Cluster-37.25795 | 14.100 | 14.610 | 14.350 | 14.520 | 14.467 | 15.363 | 14.568 | 0.427 | 0.029 | Nuclear transcription factor Y subunit A-9 OS = Arabidopsis thaliana OX = 3702 GN = NFYA9 PE = 1 SV = 1 |
3 | Cluster-37.19919 | 6.660 | 6.237 | 6.800 | 6.440 | 6.607 | 6.403 | 6.524 | 0.203 | 0.031 | bZIP transcription factor 16 OS = Arabidopsis thaliana OX = 3702 GN = BZIP16 PE = 1 SV = 1 |
4 | Cluster-37.24007 | 15.807 | 15.080 | 16.027 | 15.877 | 16.447 | 15.250 | 15.748 | 0.506 | 0.032 | Scarecrow-like transcription factor PAT1 OS = Arabidopsis thaliana OX = 3702 GN = PAT1 PE = 1 SV = 1 |
5 | Cluster-37.25129 | 14.923 | 15.803 | 15.623 | 16.607 | 16.413 | 16.583 | 15.992 | 0.666 | 0.042 | SWI/SNF complex subunit SWI3C OS = Arabidopsis thaliana OX = 3702 GN = SWI3C PE = 1 SV = 1 |
6 | Cluster-37.20455 | 14.210 | 12.717 | 12.903 | 13.020 | 13.097 | 12.807 | 13.126 | 0.549 | 0.042 | PRA1 family protein A1 OS = Arabidopsis thaliana OX = 3702 GN = PRA1A1 PE = 2 SV = 1 |
7 | Cluster-37.22273 | 28.917 | 31.807 | 29.187 | 31.723 | 31.433 | 30.167 | 30.539 | 1.297 | 0.042 | Ubiquitin carboxyl-terminal hydrolase 3 OS = Arabidopsis thaliana OX = 3702 GN = UCH3 PE = 2 SV = 1 |
8 | Cluster-37.23691 | 37.053 | 36.873 | 37.763 | 40.150 | 40.320 | 40.073 | 38.706 | 1.645 | 0.043 | FACT complex subunit SSRP1 OS = Catharanthus roseus OX = 4058 GN = SSRP1 PE = 2 SV = 1 |
9 | Cluster-37.25912 | 12.693 | 12.177 | 12.490 | 11.497 | 11.533 | 11.667 | 12.009 | 0.516 | 0.043 | Probable polyamine transporter At3g19553 OS = Arabidopsis thaliana OX = 3702 GN = At3g19553 PE = 3 SV = 1 |
10 | Cluster-37.24280 | 14.343 | 12.670 | 13.200 | 13.657 | 13.727 | 13.147 | 13.457 | 0.580 | 0.043 | U-box domain-containing protein 62 OS = Arabidopsis thaliana OX = 3702 GN = PUB62 PE = 2 SV = 1 |
11 | Cluster-37.17383 | 15.143 | 14.870 | 13.620 | 14.190 | 14.247 | 13.670 | 14.290 | 0.618 | 0.043 | Rho GTPase-activating protein 1 OS = Arabidopsis thaliana OX = 3702 GN = ROPGAP1 PE = 2 SV = 1 |
12 | Cluster-37.19705 | 15.343 | 16.090 | 14.783 | 14.757 | 14.503 | 14.267 | 14.957 | 0.661 | 0.044 | Protein IWS1 homolog 1 OS = Arabidopsis thaliana OX = 3702 GN = IWS1 PE = 1 SV = 1 |
13 | Cluster-37.22984 | 2.410 | 2.220 | 2.333 | 2.350 | 2.177 | 2.157 | 2.274 | 0.104 | 0.046 | Ubiquitin receptor RAD23c OS = Arabidopsis thaliana OX = 3702 GN = RAD23C PE = 1 SV = 2 |
14 | Cluster-37.22414 | 77.980 | 75.553 | 71.753 | 81.747 | 75.687 | 79.867 | 77.098 | 3.550 | 0.046 | 26 S proteasome non-ATPase regulatory subunit 4 homolog OS = Arabidopsis thaliana OX = 3702 GN = RPN10 PE = 1 SV = 1 |
15 | Cluster-37.24426 | 18.437 | 18.660 | 17.610 | 20.007 | 17.850 | 18.147 | 18.452 | 0.851 | 0.046 | NF-X1-type zinc finger protein NFXL1 OS = Arabidopsis thaliana OX = 3702 GN = NFXL1 PE = 1 SV = 1 |
16 | Cluster-37.11666 | 9.630 | 10.383 | 9.110 | 9.810 | 9.753 | 9.287 | 9.662 | 0.447 | 0.046 | ADP-ribosylation factor-like protein 5 OS = Dictyostelium discoideum OX = 44,689 GN = arl5 PE = 3 SV = 1 |
17 | Cluster-37.32890 | 8.513 | 8.200 | 8.537 | 7.767 | 8.917 | 8.583 | 8.419 | 0.393 | 0.047 | NR:CBFB_NFYA domain-containing protein [Cephalotus follicularis] |
18 | Cluster-37.22646 | 58.323 | 53.777 | 60.797 | 60.480 | 60.443 | 57.083 | 58.484 | 2.732 | 0.047 | Homogentisate solanesyltransferase, chloroplastic OS = Arabidopsis thaliana OX = 3702 GN = HST PE = 1 SV = 1 |
19 | Cluster-37.17807 | 15.470 | 16.177 | 16.093 | 17.560 | 16.113 | 17.137 | 16.425 | 0.771 | 0.047 | Transcription factor TGAL1 OS = Oryza sativa subsp. japonica OX = 39,947 GN = TGAL1 PE = 1 SV = 2 |
20 | Cluster-37.18684 | 3.290 | 3.660 | 3.203 | 3.383 | 3.277 | 3.407 | 3.370 | 0.160 | 0.048 | NADPH: adrenodoxin oxidoreductase, mitochondrial OS = Arabidopsis thaliana OX = 3702 GN = MFDR PE = 1 SV = 1 |
21 | Cluster-37.22228 | 14.363 | 15.527 | 13.907 | 15.467 | 14.283 | 14.083 | 14.605 | 0.709 | 0.049 | Homeobox-DDT domain protein RLT2 OS = Arabidopsis thaliana OX = 3702 GN = RLT2 PE = 1 SV = 1 |
22 | Cluster-37.22252 | 33.727 | 36.930 | 32.400 | 35.180 | 34.867 | 36.793 | 34.983 | 1.753 | 0.050 | Two-component response regulator ORR21 OS = Oryza sativa subsp. indica OX = 39,946 GN = RR21 PE = 3 SV = 1 |
23 | Cluster-37.26541 | 24.613 | 23.870 | 22.157 | 24.343 | 25.067 | 25.813 | 24.311 | 1.245 | 0.051 | NAD(P)H-quinone oxidoreductase subunit N, chloroplastic OS = Arabidopsis thaliana OX = 3702 GN = ndhN PE = 1 SV = 1 |
24 | Cluster-37.26945 | 13.093 | 13.390 | 11.563 | 12.700 | 13.070 | 13.150 | 12.828 | 0.658 | 0.051 | Histone deacetylase 6 OS = Arabidopsis thaliana OX = 3702 GN = HDA6 PE = 1 SV = 1 |
25 | Cluster-37.23473 | 33.413 | 33.263 | 32.567 | 31.783 | 30.127 | 35.113 | 32.711 | 1.681 | 0.051 | SMR domain-containing protein At5g58720 OS = Arabidopsis thaliana OX = 3702 GN = PIPC PE = 1 SV = 1 |
26 | Cluster-37.22897 | 16.893 | 18.170 | 16.800 | 18.580 | 17.427 | 16.220 | 17.348 | 0.892 | 0.051 | GATA transcription factor 24 OS = Arabidopsis thaliana OX = 3702 GN = GATA24 PE = 1 SV = 2 |
27 | Cluster-37.30730 | 39.157 | 35.600 | 33.857 | 36.707 | 37.890 | 35.527 | 36.456 | 1.885 | 0.052 | Eukaryotic translation initiation factor 4B3 OS = Arabidopsis thaliana OX = 3702 GN = EIF4B3 PE = 1 SV = 1 |
28 | Cluster-37.18046 | 12.923 | 13.167 | 11.720 | 12.807 | 12.803 | 11.667 | 12.514 | 0.650 | 0.052 | ATP-dependent RNA helicase eIF4A OS = Vanderwaltozyma polyspora (strain ATCC 22028/DSM 70294/BCRC 21397/CBS 2163/NBRC 10782/NRRL Y-828 /UCD 57 − 17) OX = 436,907 GN = TIF1 PE = 3 SV = 1 |
29 | Cluster-37.25359 | 14.157 | 12.697 | 12.307 | 13.000 | 13.223 | 12.390 | 12.962 | 0.682 | 0.053 | U-box domain-containing protein 4 OS = Arabidopsis thaliana OX = 3702 GN = PUB4 PE = 1 SV = 3 |
30 | Cluster-37.22066 | 26.233 | 27.820 | 25.603 | 29.523 | 28.487 | 27.003 | 27.445 | 1.456 | 0.053 | Polyadenylate-binding protein RBP45 OS = Nicotiana plumbaginifolia OX = 4092 GN = RBP45 PE = 1 SV = 1 |
31 | Cluster-37.23838 | 12.840 | 13.047 | 11.790 | 13.833 | 13.283 | 13.450 | 13.041 | 0.701 | 0.054 | Probable D-2-hydroxyglutarate dehydrogenase, mitochondrial OS = Oryza sativa subsp. indica OX = 39,946 GN = D2HGDH PE = 3 SV = 1 |
32 | Cluster-37.11295 | 3.080 | 2.797 | 2.703 | 2.753 | 2.663 | 2.720 | 2.786 | 0.151 | 0.054 | F-box/kelch-repeat protein At1g57790 OS = Arabidopsis thaliana OX = 3702 GN = At1g57790 PE = 2 SV = 1 |
33 | Cluster-37.24801 | 14.990 | 13.000 | 13.390 | 13.883 | 13.017 | 13.693 | 13.662 | 0.741 | 0.054 | NR: hypothetical protein EZV62_015438 [Acer yangbiense] |
34 | Cluster-37.24229 | 31.503 | 31.393 | 31.533 | 32.330 | 27.843 | 32.437 | 31.173 | 1.692 | 0.054 | GATA transcription factor 24 OS = Arabidopsis thaliana OX = 3702 GN = GATA24 PE = 1 SV = 2 |
35 | Cluster-37.24001 | 16.793 | 18.153 | 17.470 | 19.220 | 19.247 | 18.750 | 18.272 | 0.992 | 0.054 | Probable E3 ubiquitin-protein ligase ARI5 OS = Arabidopsis thaliana OX = 3702 GN = ARI5 PE = 2 SV = 1 |