Table 3 The expression stability of candidate reference genes from three software analyses.
Rank | Genorm | Normfinder | BestKeeper | Delta CT | Comprehensive ranking | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Gene | Stability | Gene | Stability | Gene | SD | CV | Gene | SD | CV | ||
1 | Cluster-37.23244 | 0.389 | Cluster-37.22228 | 0.277 | Cluster-37.22066 | 0.64 | 0.02 | Cluster-37.24280 | 2.57 | 0.08 | Cluster-37.22228 |
2 | Cluster-37.24426 | 0.389 | Cluster-37.22897 | 0.407 | Cluster-37.22897 | 0.53 | 0.02 | Cluster-37.22066 | 2.99 | 0.08 | Cluster-37.22646 |
3 | Cluster-37.24001 | 0.515 | Cluster-37.22646 | 0.734 | Cluster-37.22273 | 0.85 | 0.03 | Cluster-37.22228 | 2.38 | 0.08 | Cluster-37.22897 |
4 | Cluster-37.22646 | 0.596 | Cluster-37.22273 | 0.853 | Cluster-37.22228 | 0.91 | 0.03 | Cluster-37.22897 | 2.51 | 0.08 | Cluster-37.23244 |
5 | Cluster-37.22228 | 0.706 | Cluster-37.24280 | 1.051 | Cluster-37.24280 | 1.22 | 0.04 | Cluster-37.24426 | 2.53 | 0.08 | Cluster-37.24426 |
6 | Cluster-37.24280 | 0.786 | Cluster-37.24001 | 1.134 | Cluster-37.22646 | 1.11 | 0.04 | Cluster-37.22273 | 2.72 | 0.09 | Cluster-37.24001 |
7 | Cluster-37.26541 | 0.881 | Cluster-37.22066 | 1.235 | Cluster-37.25912 | 1.24 | 0.04 | Cluster-37.24001 | 2.51 | 0.09 | Cluster-37.22273 |
8 | Cluster-37.24801 | 0.983 | Cluster-37.24426 | 1.422 | Cluster-37.25129 | 1.83 | 0.05 | Cluster-37.22646 | 2.37 | 0.09 | Cluster-37.24280 |
9 | Cluster-37.22897 | 1.104 | Cluster-37.25912 | 1.423 | Cluster-37.23244 | 1.48 | 0.05 | Cluster-37.23244 | 2.53 | 0.09 | Cluster-37.22066 |
10 | Cluster-37.22273 | 1.207 | Cluster-37.23244 | 1.435 | Cluster-37.24001 | 1.57 | 0.05 | Cluster-37.25129 | 3.33 | 0.09 | Cluster-37.24801 |
11 | Cluster-37.22066 | 1.352 | Cluster-37.25129 | 2.005 | Cluster-37.24426 | 1.60 | 0.05 | Cluster-37.24801 | 2.96 | 0.10 | Cluster-37.25912 |
12 | Cluster-37.25129 | 1.525 | Cluster-37.24801 | 2.110 | Cluster-37.24801 | 1.79 | 0.06 | Cluster-37.25795 | 3.75 | 0.11 | Cluster-37.26541 |
13 | Cluster-37.25912 | 1.710 | Cluster-37.26541 | 2.147 | Cluster- 37.23838 | 2.77 | 0.07 | Cluster-37.25912 | 3.34 | 0.12 | Cluster-37.25129 |
14 | Cluster-37.25795 | 1.905 | Cluster-37.25795 | 2.167 | Cluster-37.25795 | 2.21 | 0.07 | Cluster-37.23838 | 5.14 | 0.12 | Cluster-37.25795 |
15 | Cluster-37.24007 | 2.177 | Cluster-37.23838 | 4.218 | Cluster-37.26541 | 1.84 | 0.07 | Cluster-37.26541 | 2.91 | 0.12 | Cluster-37.24007 |
16 | Cluster-37.23838 | 2.499 | Cluster-37.24007 | 4.369 | Cluster-37.24007 | 2.43 | 0.08 | Cluster-37.24007 | 4.82 | 0.16 | Cluster-37.23838 |
17 | Cluster-37.32890 | 3.599 | Cluster-37.32890 | 11.740 | Cluster-37.32890 | 8.01 | 0.30 | Cluster-37.32890 | 11.85 | 0.41 | Cluster-37.32890 |