Fig. 2

KRT1 was the primary contributor of green autofluorescence. Compared with normal renal tissues, the mRNA level (A) and protein level of the KRT1 (B-C) in cancerous tissues were significantly decreased (N = 4, *P < 0.05, **P < 0.01, Paired Samples t-test). Compared with HK-2 cells, the mRNA level (D) and protein level of the KRT1 (E-F) in 786-O cells were significantly decreased (*P < 0.05, **P < 0.01, Independent Samples t-test). A KRT1-silenced HK-2 cell lines (shRNA-KRT1-HK-2) was constructed. Compared to the control group, the mRNA (G) and protein levels (H-I) of KRT1 in shRNA-KRT1-HK-2 cells were significantly reduced (**P < 0.01, ***P < 0.001, Paired Samples t-test). (J/K) Compared with the control group, the green autofluorescence intensity of shRNA-KRT1-HK-2 cells was significantly decreased (**P < 0.01, Paired Samples t-test). Scale bar = 100 μm.