Fig. 3

PGRP-S promotes migration, invasion and metastasis of HCC cells. (A, B) HepG2 and SMMC7721 cells were transfected with siControl (NC) or siRNA targeting PGRP-S (Si-PGRP-S) for 48 h. The migratory and invasive abilities of PGRP-S-interfered cells were examined by wound healing assay (A) and transwell invasion assay (B). (C, D) Huh7 and MHCC97H cells were transfected with Lentivirus for PGRP-S overexpression (LV-PGRPs) and negative control lentivirus (NC). The migratory and invasive abilities of PGRP-S-interfered cells were examined by wound healing assay (C) and transwell invasion assay (D). PGRP-overexpressing Huh7 cells (LV-PGRP-S) and the control Huh7 cells (NC) were inoculated into the tail vein of BABL/c nude mice (n = 4). (E). Representative metastatic tumors in lung tissues at week 8 were shown (left panel). Representative HE staining images of lung tissue were observed under microscope (right panel). *P < 0.05; **P < 0.005; ***P < 0.001; ***P < 0.0001.