Fig. 6
From: PGRP-S promotes hepatocellular carcinoma progression via MAPK/ERK pathway by interaction with TTC1
PGRP-S-mediated cell proliferation, migration and invasion are dependent on TTC1 in HCC cells. (A, B) The cell proliferation levels were analyzed in Huh7 and MHCC97H cells transfected with Vector (NC), stably overexpressing PGRP-S (LV-PGRPs) or LV-PGRPs + Si-TTC1-1, LV-PGRPs + Si-TTC1 -2 by CCK-8 assay. (C) Indicated cells were subjected to colony formation assay. Representative images of colonies were shown and the number of colonies were counted. (D, E) Cell cycle assay was performed by flow cytometry in indicated cells. Representative images of cell cycle were shown and the percentage of G1, S and G2 stages were calculated. (F, G) The migratory and invasive abilities were examined by wound healing assay and transwell invasion assay in indicated cells. *P < 0.05; **P < 0.005; ***P < 0.001; ***P < 0.0001.
