Fig. 5 | Scientific Reports

Fig. 5

From: In silico and in vitro investigations reveal pan-PPAR agonist activity and anti-NAFLD efficacy of polydatin by modulating hepatic lipid-energy metabolism

Fig. 5

Polydatin up-regulates fatty acid metabolizing enzymes’ gene expression in HepG2 cells. Steatotic HepG2 cells were treated for 24 h with Silymarin (30µM) and Polydatin at 30µM. Figures (a) PPARα, (b) PPARβ/δ, (c) PPARγ, (d) ACC1 (e) FASN, (f) SCD1, (g) GLUT2. Each bar represents the mean ± SD. Statistical comparisons are with the steatotic control group. The results are expressed as fold-change calculated by the relative Ct method, using ß-actin as the internal control (housekeeping gene). Values are expressed as mean ± S.D. of a minimum of three experiments done in triplicate. Symbols **, ***, and **** represent statistical significance at p ≤ 0.01, 0.001, and 0.0001 respectively, and ns implies statistically not significant.

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