Fig. 2
From: Loss of TRPV4 is insufficient to promote repair in a spinal cord injury contusion model
Chimerism of microglia-like cells in the spinal cord after bone marrow transplantation and PLX5622 treatment. (A) Timeline of the experimental procedures. 10-week-old Trpv4 WT and Trpv4 KO mice receive bone marrow of Trpv4 WT Cx3cr1eGFP/+ or Trpv4 KO Cx3cr1eGFP/+ animals and PLX5622 treatment for microglia depletion in the brain and spinal cord (1.2 g/kg chow). (B) Overview of eGFP+-repopulating donor phagocytes (eGFP), IBA-1+ donor and recipient phagocytes (IBA-1), and an overlay (merge) in a sagittal spinal cord 13 weeks post-BMT (no spinal cord injury). (C) Quantification of the repopulation of Trpv4 WT (n = 9 mice) and Trpv4 KO (n = 14 mice) phagocytes in the spinal cord 13 weeks post-BMT (double-positive cells). Mann-Whitney U test. Data are presented as means ± SEM. Scale bars: 500 μm. BMT, bone marrow transplantation; WT, wild-type; KO, knockout; eGFP, enhanced green fluorescent protein; WBI, whole body irradiation; dpi, days post-injury.
