Fig. 3
From: Loss of TRPV4 is insufficient to promote repair in a spinal cord injury contusion model
Recovery after spinal cord injury does not improve in a phagocyte-specific bone marrow transplantation model. (A) Functional recovery over 7 days after contusion injury at T8 of the four different BMT groups: (1) n = 5, (2) n = 7, (3) n = 2, and (4) n = 4, of 3 individual experiments. Overview of bone marrow chimera groups: (1) full WT (Trpv4 WT Cx3cr1eGFP/+ → Trpv4 WT); (2) phagocyte-specific Trpv4 KO (Trpv4 KO Cx3cr1eGFP/+ → Trpv4 WT); (3) full Trpv4 KO (Trpv4 KO Cx3cr1eGFP/+ → Trpv4 KO); (4) TRPV4-expressing phagocytes in a TRPV4-deficient host (Trpv4 WT Cx3cr1eGFP/+ → Trpv4 KO). (B) IBA-1+ phagocytes in sagittal spinal cord sections at 7 dpi of all BMT groups, with an overview of the lesion and marked regions at different distances from the lesion center. (C) Quantification of the phagocytic density at different distances from the lesion center at 3 and 7 dpi. Two-way ANOVA (A) and Multiple Mann-Whitney U test (C). Data are presented as means ± SEM. Scale bar: 500 μm. T8, thoracic level 8; BMT, bone marrow transplantation; WT, wild-type; KO, knockout; eGFP, enhanced green fluorescent protein; BMS, Basso Mouse Scale; dpi, days post-injury; IMV, intensity mean value.
