Fig. 3
Characterization of the sympathoadrenal neuronal lineage in the carotid body. (A) UMAP plot highlighting the three cell clusters that define the sympathoadrenal neuronal lineage within the carotid body. (B) UMAP plots showing the expression of selected genes to distinguish glomus cells from neuroblasts. (C) UMAP embedding plot illustrating the dopaminergic differentiation score for each cell. The color gradient represents the expression intensity of the differentiation gene signature. Progenitor cells exhibit the lowest differentiation scores, while glomus cells show the highest, with neuroblasts in an intermediate state. (D) Violin plot visualization of the dopaminergic differentiation score, with significant p-values indicated. (E) UMAP plots displaying genes highly correlated with the differentiation trajectory, either positively (upregulated; top) or negatively (downregulated; bottom). (F) Correlation analysis between the dopaminergic differentiation score and other gene expression signatures, including "Neural Crest Cell Differentiation" (from the Gene Ontology database) and "O₂-sensing" (defined in this work).
