Fig. 5
Single-cell transcriptomic analysis of the hypoxic carotid body. (A) UMAP embedding of 18,586 single cells from hypoxic rat carotid bodies. Transcriptionally similar cell clusters are color-coded and labeled by cell type. (B) Comparison of cell cluster frequencies between carotid bodies obtained from rats maintained in standard conditions (21% O2 atmosphere: normoxia, Nx) or after 10 days in a chamber with 10% O2 tension (hypoxia, Hx). Note how neuroblasts disappear in hypoxia, while glomus and blood cells clearly increase their proportions within the tissue. (C) Violin plots showing the expression of specific sympathoadrenal markers in hypoxic carotid body cells. Schwann cell, endothelial cell and blood cell clusters have been excluded for clarity. (D) UMAP plots depicting the expression of selected sympathoadrenal markers (see Fig. 1B) in mesenchymal, neuronal, and progenitor cell clusters within the hypoxic carotid body.
