Fig. 1
From: Cell-type specific effects of the long non-coding RNA HIF1A-AS3 on HIF1A expression in kidney cells
Gene locus of HIF1A mRNA, HIF1A-AS1, AS2, and AS3 lncRNA and their expression in HK-2 cells. (A) Modified depiction of the gene locus of HIF1A mRNA, HIF1A-AS1, AS2, and AS3 (all blue) as provided by UCSC genome browser based on current RefSeq database (Annotation Release NCBI RefSeq GCF_000001405.40-RS_2023_10). (B) Using the UCSC genome browser tool “costum tracks”, data for HIF1A-AS3 variants from Ensembl genome browser (release 112) are shown in red. (C) Illustration of RACE result (yellow) for HIF1A-AS3 sequence analyses was also conducted with “costum tracks” tool. (D) A selection of alignments of 17 species (green) compared to human genome assembly is shown, applying the UCSC track Cactus Alignment & Conservation of Zoonomia Placental Mammals (241 Species). Grey bars highlight the exon positions of HIF1A-AS1, AS2, and AS3. For better clarity, genome positions Chr14:61,682,000–61,695,000 and Chr14:61,700,000–61,706,000 were omitted, marked by 3 black dots. (E) RNA expression levels of HIF1A, HIF1A-AS1, AS2, or AS3 in untreated HK-2 cells determined by qPCR, depicted as logarithmic plot. Dots and triangles represents single results, bars the mean expression. CT: threshold cycle; n = 5–6.
