Fig. 3
From: Cell-type specific effects of the long non-coding RNA HIF1A-AS3 on HIF1A expression in kidney cells
Expression of HIF1A mRNA, HIF1A-AS2, and 3 lncRNA, and Hif1α protein after knockdown (KD) or overexpression (OE) of HIF1A mRNA, HIF1A-AS1, AS2, and AS3 lncRNA, respectively in HK-2 cells under normal glucose (NG) or high glucose (HG), respectively, under normoxia (NOX) or hypoxia (HOX). Efficiency of KD of HIF1A mRNA (A), HIF1A-AS2 lncRNA (B), and HIF1A-AS3 lncRNA (C) and the corresponding effect (indicated by arrow) on HIF1A mRNA and HIF1A-AS2 or AS3 lncRNA expression shown as xfold change (xFC). Data are represented by bars + standard deviation (SD). (D) Detection of Hif1α by western blotting after KD of HIF1A-AS1, AS2 and AS3, respectively, under NG or HG and NOX or HOX. Scrambled siPools served as negative control (NC). Thereunder whole protein used for normalization. (E) Quantification of Hif1α based on band density and size after total protein normalization after KD of HIF1A-AS3 under NG or HG and NOX or HOX. The whole protein stain free western blot image is compressed in length. Four original immunoblotting images were cropped and merged to with a white space between each blot. Scrambled siPools served as NC. xFCs are represented by bars + SD. (F) Efficiency of OE of HIF1A, HIF1A-AS1, and HIF1A-AS3 lncRNA depicted as xFC. Data are represented by bars + SD. n = 3–4. Significance was analyzed by ANOVA, followed by Student’s t-test. *p < 0.05; **p < 0.001; ***p < 0.00001 compared to NG + NOX.
