Fig. 5
From: Cell-type specific effects of the long non-coding RNA HIF1A-AS3 on HIF1A expression in kidney cells
Rescue assays to characterize potential mutual dependencies in expression of HIF1A, HIF1A-AS2 or AS3 in HK-2 cells under basal conditions. The upper panel (red bars) shows x-fold change (xFC) of HIF1A, the second (yellow bars) or third panel (blue bars) display xFC of HIF1A-AS2 and AS3, respectively. As depicted below the third panel, experiments were conducted with “NC (KD)” as knockdown (KD) with scrambled siPools as negative control (NC) and “KD HIF1A”, “KD HIF1A-AS2”, or “KD HIF1A-AS3” as KD for the corresponding RNA. After each KD, an overexpression (OE) experiment was performed using either an empty pcDNA vector as NC “NC (OE)” or a pcDNA carrying the sequence of HIF1A, HIF1A-AS2 or AS3: “OE HIF1A”, “OE HIF1A-AS2”, or “OE HIF1A-AS3”. n = 4. Significance was analyzed by ANOVA, followed by Student’s t-test. *p < 0.05; **p < 0.001; ***p < 0.00001 compared to NC or as otherwise specified.
