Fig. 7
From: Cell-type specific effects of the long non-coding RNA HIF1A-AS3 on HIF1A expression in kidney cells
Expression of Hif1α protein, HIF1A mRNA, HIF1A-AS1, and AS3 lncRNA in hMCs after stimulation with high mannitol (HM), and high glucose (HG), respectively, under normoxia (NOX) or hypoxia (HOX) as well as knockdown (KD) and overexpression (OE) experiments. (A) RNA expression levels of HIF1A, HIF1A-AS1, AS2, or AS3 in untreated hMCs determined by qPCR, depicted as logarithmic plot. Dots and triangles represents single results, bars the mean expression. CT: threshold cycle. (B) X-fold changes (xFC) of HIF1A (blue) and HIF1A-AS3 (red) RNA expression under normal glucose (NG), high mannitol (HM) as osmotic control, and high glucose (HG), respectively. Cells were simultansouly exposed to normoxia or hypoxia. Data are represnted by bars + standard deviation (SD). (C) OE efficiency of HHIF1A-AS3 lncRNA and the corresponding effect (indicated by arrow) on HIF1A mRNA expression shown as xFC with bars + SD. (D) Detection of Hif1α by western blotting after OE of HIF1A-AS3 under NG or HG and NOX or HOX. Scrambled siPools or empty vector served as negative control (both NC); basal: untreated cells. Thereunder whole protein used for normalization and quantification of Hif1α based on band density and size after total protein normalization shown as xFCs, The whole protein stain free western blot image is compressed in length. Four original immunoblotting images were cropped and merged to with a white space between each blot. xFCs are represented by bars + SD represented by bars + SD. n = 3 Significance was analyzed by ANOVA, followed by Student’s t-test. *p < 0.05; **p < 0.001 compared to NG.
