Fig. 3
KHDRBS1- and DDX5-knockout HeLa cells exhibit increased sensitivity to F1F3 treatment. (A-C) Dose-dependent cytotoxicity of F1, F3, and F1F3 polypeptides in wild-type HeLa cells, as measured by optical density (OD540 nm). (D-F) Cytotoxicity assays in KHDRBS1-KO HeLa cells treated with F1, F3, or F1F3 showed enhanced sensitivity, indicated by a greater reduction in OD540 nm. (G-I) Similar dose-dependent cytotoxicity was observed in DDX5-KO HeLa cells. (J-L) Calculated IC₅₀ values for F1, F3, and F1F3 across wild-type, KHDRBS1-KO, and DDX5-KO HeLa cells demonstrated increased potency of the peptides in KO cell lines. (M) Real-time fluorescence imaging of KHDRBS1-KO HeLa cells within 2 h post-F1F3 treatment revealed dynamic cellular responses. (A-L) Data are presented as mean ± SEM. Statistical significance was evaluated using one-way ANOVA: ns (not significant), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
