Fig. 6
TP53 and its mutants regulate cell proliferation and lncRNA expression in DLD1 cells. (a) CCK8 assay the growth of TP53-null control cells and TP53-WT cells in 7 days. Data are presented as mean ± STDEV; biological replicates n = 3, error bars: standard deviation. 2-way ANOVA test is applied, *** indicates P < 0.001. (b) CCK8 assay the growth of TP53-R175H cells and TP53-WT cells in 7 days, ** indicates P < 0.01, *** indicates P < 0.001. (c) CCK8 assay the growth of TP53-R175P cells and TP53-WT cells in 7 days, *** indicates P < 0.001. (d) ChIP-qPCR analyses of TP53 binding to the promoter of lncRNA H19 and lncRNA LINC00969, respectively. TP53-WT DLD1 cells were used. Enrich folds indicate the ratio of immunoprecipitation (IP) versus input in qPCR assay. Data are presented as mean ± STDEV. Student t-test is applied, *** indicates P< 0.005, **** indicates P < 0.001.
