Figure 2

Cytokine profiling of conditioned media from PEO1 and PEO4 cells. PEO1 and PEO4 cells were treated with IFNγ, stattic, or both in combination for 24 h. Conditioned media were collected to determine the levels of 36 cytokines using a cytokine immunoarray. The levels of representative cytokines produced by PEO1 and PEO4 cells are shown (A). Conditioned media of PEO1 and PEO4 cells under normal growth conditions after 24 h were collected to determine the levels of 105 cytokines using a cytokine immunoarray. The levels of cytokines over-produced by PEO1 cells (B) or by PEO4 cells (C) were shown. PEO1 and PEO4 cells produced comparable levels of MIF, which are also demonstrated. Data are means ± SD (n = 2) of duplicate measurements from a representative experiment.