Figure 3 | Scientific Reports

Figure 3

From: Correction: CXCL10-induced regulatory T cells and adenosine signaling promote immunosuppression and progression of epithelial ovarian cancer

Figure 3

Immunosuppressive effects of PEO1 and PEO4 cells on PBMCs and SUPT1 cells. PBMCs or SUPT1 cells activated by CD3/CD28 antibodies were co-cultured with PEO1 or PEO4 cells for 48 h. PBMCs were collected, stained, and analyzed by a flow cytometer to determine the percentage of the CD8 + CD25 + T cell population in PBMCs (A) and in SUPT1 cells (B), as well as the percentage of the CD4 + CD25 + T cell population in PBMCs (C). To determine the level of the Treg population, PBMCs were first gated for CD4 + cells to determine the percentage of the CD4 + T cell population in PBMCs (Top). The CD4 + T cell population was subsequently gated for FXOP3 + CD25 + cells to determine the percentage of FOXP3 + CD25 + T cells in the CD4 + T cell population (bottom). The bar graph shows the percentage of FOXP3 + CD4 + CD25 + T cells in PBMCs by calculating FOXP3 + CD25 + T cells of CD4 + T cells of total PBMCs (D). Data are means ± SD (n = 3) from three independent experiments.

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