Fig. 3

RvD1 restores capillary growth impairment induced by Bleo. The left-hand panels show representative immunostaining of the vascular endothelial cells as an index of vascularization in capillaries < 20 µm in diameter (a) and small vessels of 20–100 µm in diameter (b). Red arrows indicate CD31-positive endothelial cells. Magnification, 100 × ; Scale bar, 100 μm. The right-hand graphs indicate the number of CD31-positive endothelial cells with diameter shown in the left in the HPF (magnification 100 ×). White bar, Control group; black bar, Bleo group; blue bar, Bleo + RvD1 group; gray bar, RvD1 alone group. Mean ± SD. N = 5–8/group. * p < 0.05, ** p < 0.01, † p < 0.001. Bleo administration significantly interfered with vascular development. RvD1 restored the number of capillary (< 20 µm in diameter) endothelial cells but did not reach the control levels. HPF, high-power field.