Fig. 3 | Scientific Reports

Fig. 3

From: Ligand binding and partial characterization of sex-specifically expressed lipocalins of submandibular and lacrimal glands of Syrian hamsters

Fig. 3

No cross-reactions with natural MSP and FLP proteins were detected in Western blots using anti-phosphoserine/threonine/tyrosine antibodies. Purified natural MSP (lane 1), purified natural FLP (lane 2), SMG extract of male hamster (lane 3) and LG extract of ovariectomized female hamster (lane 4) were run in 12% SDS-PAGE in three similar sets with each containing the above mentioned 4 samples (total 12 lanes). A cut segment of the gel containing one set (with 4 lanes) was Coomassie stained (Panel A) while the remaining gel was blotted. Transfer of proteins after blotting was confirmed by visualizing after Ponceau staining (images of Ponceau-stained blots were not recorded). The blot was then appropriately cut into 2 similar sets containing 4 lanes each, which were kept for blocking and one set was probed with antibody against phosphoserine/threonine/tyrosine (Panel B) while the other set was probed with a separate antibody against phosphotyrosine (Panel C). See “Methods” section for details.

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