Fig. 6

Qualitative immunofluorescent images of cannabinoid treatment on Liver-Chips. a) Oxidative stress assessment of Liver-Chips on day 7 post-treatment. All Chips were stained for CellRox (red), and nuclei were stained with DAPI (blue). Scale bar represents 100 µm. Dose-dependent increases in ROS and decreases in NPC count were observed in response to CBC or APAP treatment. All other treated hepatocytes had ROS expression like the vehicle, whereas all cannabinoid treated NPCs had increased ROS expression as compared to the vehicle. b) Mitochondrial function assessment of Liver-Chips on day 7 post-treatment. All Chips were stained for Mitotracker (yellow), and nuclei were stained with DAPI (blue). Scale bar represents 100 µm. APAP, CBN or CBC caused dose-dependent decreases in Mitoracker signal hepatocytes and NPCs. CBG also caused decreases in NPC expression of Mitotracker, whereas all other treatments trended like the control.