Fig. 5
(A) Representative confocal images of Iba1 staining in the dentate gyrus of B6 wild-type mice and FIRE mice. Scale bar, 50 μm. (B) Quantification of brain weight at various time points in B6 and FIRE animals overexpressing wild-type tau compared to non-coding control vector. 6wk B6 AAV-STOP, N = 10; 6wk B6 AAV-Tau, N = 17; 6wk FIRE AAV-STOP, N = 6; 6wk FIRE AAV-Tau, N = 7; 12wk B6 AAV-STOP, N = 24; 12wk B6 AAV-Tau, N = 21; 12wk FIRE AAV-STOP, N = 10; 12wk FIRE AAV-Tau, N = 14. One-way ANOVA and Kruskal-Wallis test were used for the 6- and 12-week time points, respectively, depending on results for normality. (C) Representative confocal images of NeuN+ CA1 neurons in B6 and FIRE mice 6 weeks post-infection with AAV-STOP or AAV-Tau. Scale bar, 50 μm. Quantification of relative neuronal density in B6 and FIRE animals overexpressing wild-type tau compared to non-coding control vector animals by region at 6 weeks of age. B6 AAV-STOP, N = 6; B6 AAV-Tau, N = 6; FIRE AAV-STOP, N = 6; FIRE AAV-Tau, N = 7. (D) Quantification of p-cJun+ neurons in brain subregions at 6 weeks of age in both B6 and FIRE mice expressing tau or control vector. B6 AAV-STOP, N = 6; B6 AAV-Tau, N = 6; FIRE AAV-STOP, N = 6; FIRE AAV-Tau, N = 7.
