Fig. 6
(A) Representative confocal images of thalamus in AAV-Tau and AAV-STOP animals at 6 weeks of age. Scale bar, 50 μm. Quantification of % HT7+ neurons in the thalamus of 6-week-old AAV-Tau animals. (B) Distribution of transgenic human tau (HT7) among neuronal subtypes, including excitatory (CamKIIα+) and inhibitory (PV+) neurons in the cortex and thalamus. Scale bar, 30 μm. (C) Quantification of γH2AX+ and p-cJun+ neurons in the B6 thalamus at 6 weeks of age in AAV-Tau compared to AAV-STOP animals. AAV-STOP, N = 6; AAV-Tau, N = 6. (D) Quantification of GFAP+ astrocytes and Iba1+ microglia in the B6 thalamus at 3 and 6 weeks of age in AAV-Tau compared to AAV-STOP animals. 3wk AAV-STOP, N = 5; 3wk AAV-Tau, N = 5; 6wk AAV-STOP, N = 6; 6wk AAV-Tau, N = 6. (E) Representative confocal images of NeuN+ neurons in thalamus, at two time points after neonatal infection with AAV-STOP or AAV-Tau. Scale bar, 50 μm. Quantification of relative thalamic neuronal density. 3wk AAV-STOP, N = 5; 3wk AAV-Tau, N = 5; 6wk AAV-STOP, N = 6; 6wk AAV-Tau, N = 6. (F,G) Quantification of relative thalamic neuronal density (F) and p-cJun+ neurons (G) at 6 weeks in FIRE animals expressing AAV-Tau compared to AAV-STOP. AAV-STOP, N = 6; AAV-Tau, N = 7. (H) Quantification of HT7+ neuron percentage in thalamus of B6 and FIRE mice expressing AAV-Tau. AAV-STOP, N = 6; AAV-Tau, N = 7.
