Fig. 2
SGs affect protein nanoparticle-related osmotic pressure in inflammasomes in U87 cells stimulated with LPS and nigericin. All U87 cells were incubated with 100 ng/mL LPS for 4 h and then with 20 µmol/L nigericin for 30 min in the presence or absence of EWSR1-G511A or TRIM21 siRNA. (A) Images taken during a 30-min time lapse of FRET analysis (calibration bar: 0.1–1.5; scale bar: 10 μm). (B) Standardized CFP/FRET ratio of vimentin at minute 30. (C) Cytoplasmic osmolarity determined on a freezing-point osmometer. (D) protein nanoparticle count rate. (E-G) cytoplasmic Ca2+ (E), Cl− (F) and K+(G). (H) Membrane potential variation. Ctrl, control; Nig, nigericin. Data are expressed as means ± SD(n = 3). *p < 0.05, **p < 0.0,.****p < 0.0001.
