Fig. 5
EWSR1 mutation prevents protein nanoparticle-related osmotic pressure recovery by inhibiting SG formation and depolymerization in U87 cells. All U87 cells were stimulated with 100 ng/mL LPS for 4 h, followed by incubation with 0.1 mmol/L H2O2 for 0, 15, 30, 45, and 60 min and then by stimulant removal and further incubation for 60 min (i.e., until 120 min after initial stimulation) in the presence or absence of EWSR1-G511A. (A and D) Standardized CFP/FRET ratio of vimentin at minutes 0, 15, 30, 45, 60, and 120 (n ≥ 3). (B and E) Cytoplasmic osmolarity determined on a freezing-point osmometer. (C and F) protein nanoparticle count rate. Ctrl, control. Data are expressed as means ± SD (n = 3). ***p < 0.001.****p < 0.0001.
