Fig. 7
Protein nanoparticle generation induced by cytoskeleton depolymerization blocks SG formation in U87 cells. All U87 cells were incubated with 0.1 mmol/L H2O2, 10 µmol/L Cyto B, and 100 µmol/L Noc for 15 min. (A) Images taken during a 15-min time lapse of FRET analysis (calibration bar: 0.1–1.5; scale bar: 10 μm). (B) Standardized CFP/FRET ratio of vimentin at minute 15 (n = 3). (C) Cytoplasmic osmolarity determined on a freezing-point osmometer. (D) protein nanoparticle count rate. (E) Normalized fluorescence intensity of Ca2+. Ctrl, control. Data are expressed as means ± SD of n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
