Fig. 7
Knockdown of OVOL1 suppresses proliferation and impairs the ERK/JNK/p38 signaling pathway in pancreatic cancer cells. (A, B) GSEA plots of enrichment of “BENPORATH_PROLIFERATION” (A) and “REACTOME_RAF_INDEPENDENT_MAPK1_3_ACTIVATION” (B) signaling pathways in OVOL1High versus OVOL1Low in TCGA pancreatic cancer cohort. (C, D) CCK-8 assay of MIAPaCa-2 (C) cells and AsPC-1 (D) transfected with siOVOL1#1, siOVOL1#2, or siCtrl. (E) Wound healing assay of MIAPaCa-2 cells transfected with siOVOL1#1, siOVOL1#2, or siCtrl. (F) Western blot analysis of the indicated proteins in MIAPaCa-2 cells transfected with siOVOL1 or siCtrl (Left panel), and the band intensities were quantified using ImageJ software (Right panel). (G) Correlation analysis of OVOL1 expression and Ki67 expression in TCGA pancreatic cancer cohort. (H) Ki67 expression in low- and high-OVOL1 pancreatic tumors in TCGA cohort. *p < 0.05, **p < 0.01, and ***p < 0.001.
