Fig. 1
From: A designer polyQ fusion protein modulates NF-κB signaling by sequestering P65/RelA into aggregates
Design of the polyQ-LDEL fusion and examination of its interaction with P65. (A) Schematic diagram of a designer polyQ fusion protein, Atx793Q-N172-LDEL. Atx793Q-N172, the N-terminal 172-residue fragment of Atx793Q. LDEL, a homologous peptide sequence, LDKALDELMDGDIIVFQK, from the UbL2 domain of USP7. (B) Examining the interaction between Atx710Q-N172-LDEL and P65 by co-IP assay. HEK 293T cells were co-transfected with FLAG-tagged Atx710Q-N172 or Atx710Q-N172-LDEL and HA-P65. At 48 h post-transfection, the cell lysates were subjected to co-IP assay using anti-FLAG agarose beads. Vec., vector; Atx710Q-N172, the N-terminal 172-residue fragment of Atx710Q; LDEL, Atx710Q-N172-LDEL, a polyQ fusion combined Atx710Q-N172 with LDEL.
