Fig. 3

Association Analysis of Palmitoylation-Related Genes with the Development of NAFLD. (A) A volcano plot was constructed to identify DEGs between normal and NAFLD samples. (B) A heatmap was generated to display the top 20 upregulated and downregulated genes, sorted by log2 fold change. (C) A Venn diagram was utilized to illustrate the intersection of genes identified through WGCNA, DEGs, and palmitoylated genes. (D) GO analysis was performed, and the results were presented in a circular plot. (E) GO analysis was further visualized in a bar chart, with annotations for biological processes (BP), cellular components (CC), and molecular functions (MF). (F) KEGG pathway enrichment analysis was conducted, and the findings were depicted in a circular plot. (G) KEGG pathway enrichment analysis was also represented in a bar chart to provide a detailed overview of the enriched pathways.