Fig. 11

MRPL21 knockdown inhibited oxidative phosphorylation and induces mitochondrial dysfunction in neuroblastoma cells. MRPL21 knockdown reduces mitochondrial oxygen consumption in SH-SY5Y (A) and SK-N-BE2 (B) cells. The oxygen consumption rate (OCR) was measured using the Seahorse XFe96 analyzer. Oligomycin, FCCP, and rotenone/antimycin A (ROT/AA) were sequentially added to assess mitochondrial function. (C) MRPL21 knockdown reduces ATP production in SH-SY5Y and SK-N-BE2 cells. MRPL21 knockdown impairs mitochondrial membrane potential in SH-SY5Y (D) and SK-N-BE2 (E) cells.